![]() Gzip: /home/app/Desktop/Untitled is a directory - ignored * Running CMD: /home/app/anaconda3/bin/STAR -genomeDir /media/app/New_Volume/jim/reference/Star-Fusion_genome_resource_lib/hg38/GRCh38_v27_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx -outReadsUnmapped None -chimSegmentMin 12 -chimJunctionOverhangMin 12 -chimOutJunctionFormat 1 -alignSJDBoverhangMin 10 -alignMatesGapMax 100000 -alignIntronMax 100000 -alignSJstitchMismatchNmax 5 -1 5 5 -runThreadN 10 -outSAMstrandField intronMotif -outSAMunmapped Within -outSAMtype BAM Unsorted -readFilesIn /home/app/Desktop/Untitled Folder/GlentisRNASeq/top_dir/GlentisS1_S17_R1_ /home/app/Desktop/Untitled Folder/GlentisRNASeq/top_dir/GlentisS1_S17_R2_ -outSAMattrRGline ID:GRPundef -chimMultimapScoreRange 10 -chimMultimapNmax 10 -chimNonchimScoreDropMin 10 -peOverlapNbasesMin 12 -peOverlapMMp 0.1 -genomeLoad NoSharedMemory -twopassMode Basic -readFilesCommand 'gunzip -c' My command is : STAR-Fusion -left_fq GlentisS1_S17_R1_ -right_fq GlentisS1_S17_R2_ -genome_lib_dir '/./GRCh38_v27_CTAT_lib_Feb092018/ctat_genome_lib_build_dir' -CPU 10 -output_dir STAR-Fusion_outdir/īut the outcome is : CMD: mkdir -p /home/app/Desktop/Untitled Folder/GlentisRNASeq/top_dir/STAR-Fusion_outdir//_starF_checkpointsĬMD: mkdir -p /home/app/Desktop/Untitled Folder/GlentisRNASeq/top_dir/STAR-Fusion_outdir//star-fusion.preliminary ![]() Also i downloaded the plug and play genome resource library so i guess thats ok. ![]() ![]() I force installed the 2.6.1d so now it works. ![]()
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